CRYOPRESERVATION OF PAPAYA SHOOT TIPS BY VITRIFICATION
نویسندگان
چکیده
منابع مشابه
Cryopreservation of in vitro-grown shoot tips of papaya (Carica papaya L.) by vitrification
Shoot tips from 4to 6-week-old in vitro grown plantlets of six different cultivars of papaya were cryopreserved by vitrification. Shoot tips were treated in a 2 ml cryo-tube filled with a solution of 2 M glycerol and 0.4 M sucrose at 25°C for 20 min, then dehydrated with 1 ml pre-cooled vitrification solution at 4°C for 60 min. Treated samples were kept in liquid nitrogen for at least one week....
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In vitro shoot tips of Capparis spiunosa were cryopreserved using the vitrification, encapsulation/ dehydration and encapsulation vitrification techniques. In the vitrification procedure, a maximum of 70% regrwoth was obtained when shoot tips were treated with the Plant vitrification solution (PVS2) for 60 min at 0°C and plunged directly into liquid nitrogen. In encapsulation dehydration, shoot...
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Shoot tips of cassava (Manihot esculenta Crantz) in vitro plantlets were successfully cryopreserved using the encapsulation-vitrification technique. Nodal cuttings of 5 mm length with one leaf were cultured on modified MS medium in Petri dishes (90 mm x 20 mm) for about 28 days. Excised shoot tips were precultured on sucrose enriched (0.3 M) medium for 16 h, encapsulated and osmoprotected with ...
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Potato is one of the most important staple crops in both developed and developing countries of the world. Potato genetic resources are a prerequisite for potato breeding in both conventional and genetic engineering programmes. Cryopreservation has long been considered an ideal means for the long-term conservation of plant genetic resources. This review provides comprehensive information on vari...
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In vitro shoot-tips of three cultivars of tropical taro (Colocasia esculenta var. esculenta (L.) Schott) were successfully cryopreserved by vitrification. Different conditioning treatments were required for each of the cultivars, while the vitrification protocol was constant for all. For the cultivars E399 and CPUK, shoot-tips from three-month-old in vitro plants grown on solidified MS were pre...
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ژورنال
عنوان ژورنال: HortScience
سال: 1990
ISSN: 0018-5345,2327-9834
DOI: 10.21273/hortsci.25.9.1066b